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Test Code 00013 Tzanck Smear, Cytology

Performing Laboratory

MaineGeneral Medical Center

Specimen Requirements

Wet lesions are more diagnostic; if lesions are dry, moisten with wet compresses for 30 minutes to soften lesion and collect specimen as follows:

1. Using a lead pencil, label frosted end of glass slide with patient’s name (first and last), date and actual time of collection, collector’s initials, and source/site of specimen.

2. Scrape lesion firmly, being sure to obtain material from base of lesion.

3. Immediately smear specimen evenly onto a glass slide using a rolling motion.

4. Fix slide immediately with cytology spray fixative or immerse in 95% alcohol  before slide dries. Hold spray about 12 inches away from slide.

5. Place slide in a slide/cardboard holder.

6. Deliver to Cytology Laboratory.

Note:  1. The following specimens will be returned to the submitting physician:

A. No requisition form

B. Name on requisition does not match name on specimen

C. Broken slide that cannot be reconstructed

D. Unlabeled specimen

E. Fixation in formalin

F. Air-drying artifact

G. Slide adhered to cardboard holder

2. Please complete and submit an “Anatomic Pathology Request Form” stock form #320-1415.

3. Place specimen in a plastic specimen bag with request form inserted into pocket separate from specimen.

Test Classification and CPT Coding

88161

Reference Values

An interpretative report will be provided.

Day(s) Test Set Up

Monday through Friday