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Test Code CDIFF Clostridium difficile Toxins A/B and glutamate dehyrogenase (GDH) antigen, with Reflex, Feces

Performing Laboratory

MaineGeneral Medical Center

Reference Values

Negative for Clostridium difficile toxins A/B

Negative for Clostridium difficile glutamate dehyrogenase (GDH) antigen by EIA

Negative for Toxigenic Clostridium difficile by LAMP DNA Amplification.

Day(s) Test Performed

Monday - Sunday

Methodology

Enzyme Immunoassay (EIA)

Amplified DNA Probe by loop-mediated isothermal DNA amplification (LAMP), if indicated.

Note: If Clostridium difficile Toxins A/B and GDH antigen is negative then a Clostridium difficile Amplified DNA Probe assay for toxigenic C.difficile will be performed at an additional charge.

Specimen Requirements

Liquid or unformed stool

Submit 5 - 10 mL of fresh, liquid stool in a tightly sealed, plastic container. Formed stool is not acceptable. Label specimen with patient’s name (first and last), date of birth, date and time of collection and collector’s initials. Store specimen on ice or at 2-8°C until tested.

Forward to laboratory within 2 hours of collection.

Test Classification and CPT Coding

87324-CDIFF toxin by EIA

87449-CDIFF antigen by EIA

87493-Infectious agent detection by nucleic acid (DNA or RNA); Clostridium difficile, toxin gene(s), amplified probe technique, if indicated