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HelpTest Code DIFF Differential Leukocyte Count, Blood
Performing Laboratory
MaineGeneral Medical Center
Specimen Requirements
Whole Blood
Specimen must arrive within 24 hours of draw.
Smear preparation on specimens older that 4 hours may exhibit a loss of cellular integrity.
Draw blood in a lavender-top (K2-EDTA) tube(s). For pediatric specimens, draw blood in a lavender-top (K2- EDTA) BD MICROTAINER® MAP microtube. Label specimen with patient’s name (first and last), date of birth, date and time of collection and the phlebotomist’s initials. Send whole blood refrigerated. Do not freeze.
*Collection tube must be filled to ½ of its intended total volume to ensure adequate blood to anticoagulant ratio.
Specimen stability: 24 hours at room temperature or kept refrigerated at 2-8°C
Minimum volume: 250 µL
Specimen rejection criteria:
Clotted blood
Samples that are > 24 hours old
Collection tubes that are less that one-half the intended total volume
Improper specimen identification
Reference Values
SEGMENTED NEUTROPHILS
Males:
0-5 years: not established
6-11 years: 29.0-66.0%
12-15 years: 28.0-78.0%
≥16 years: 42.0-75.0%
Females:
0-5 years: not established
6-11 years: 28.0-68.0%
12-15 years: 33.0-77.0%
≥16 years: 42.0-75.0%
BAND NEUTROPHILS
Males:
0-5 years: not established
6-11 years: 0.0-2.0%
≥12 years: 0.0-3.0%
Females:
0-5 years: not established
≥6 years: 0.0-3.0%
LYMPHOCYTES
Males:
0-5 years: not established
6-11 years: 23.0-64.0%
12-15 years: 18.0-54.0%
≥16 years: 16.0-52.0%
Females:
0-5 years: not established
6-11 years: 25.0-64.0%
12-15 years: 20.0-58.0%
≥16 years: 16.0-52.0%
MONOCYTES
Males:
0-5 years: 3.0-10.0%
6-11 years: 1.0-13.0%
12-15 years: 1.0-12.0%
≥16 years: 1.0-11.0%
Females:
0-5 years: 3.0-10.0%
6-11 years: 1.0-10.0%
≥12 years: 1.0-11.0%
EOSINOPHILS
Males:
0-5 years: 0.0-8.0%
6-11 years: 0.0-11.0%
12-15 years: 0.0-9.0%
≥16 years: 0.0-7.0%
Females:
0-5 years: 0.0-8.0%
6-11 years: 0.0-11.0%
12-15 years: 0.0-8.0%
≥16 years: 0.0-7.0%
BASOPHILS
Males:
0-11 years: 0.0-3.0%
12-15 years: 0.0-2.0%
≥16 years: 0.0-4.0%
Females:
0-15 years: 0.0-3.0%
≥16 years: 0.0-4.0%
METAMYELOCYTES
0-5 years: not established
6-15 years: 0.0-0.0%
≥16 years: <1.0%
MYELOCYTES
0-5 years: not established
6-15 years: 0.0-0.0%
≥16 years: <0.5%
Day(s) Test Performed
Monday through Sunday
Methodology
Manual—Microscopic Examination of Cells
Includes segmented neutrophils, neutrophilic bands, lymphocytes, monocytes, eosinophils, basophils, erythrocyte morphology, and platelets.
Test Classification and CPT Coding
85007