Specimen Requirement

1. Collect specimen using one of the following devices:

 a. Broom-like Protocol- Obtain an adequate sampling. If desired, use lukewarm water to warm and lubricate the speculum. Insert the central bristles of the broom into the endocervical canal deep enough to allow the shorter bristles to fully contact the ectocervix. Push gently, and rotate the broom in a clockwise direction five (5) times. Rinse the broom as quickly as possible into the PreservCyt Solution vial by pushing the broom into the bottom of the vial ten (10) times, forcing the bristles apart. As a final step, swirl the broom vigorously to further release material. Discard the collection device.

 b. Brush/Spatula Endocervical protocol- Select the contoured end of plastic spatula and toate it 360 degrees around the entire exocervix while maintaining tight contact with exocervival surface. Rinse the spatula as quickly as possible into the PreservCyt Solution vial by swirling the spatula vigorously in the vial ten (10) times. Discard the spatula. Obtain an adequate sampling from the endocervix using the endocervical brush. Insert the brush into the cervix until only the bottom-most fibers are exposed. Slowly rotate 1/4 or 1/2 turn in one direction. DO NOT OVER ROTATE. Rinse the brush as quickly as possible in the PreservCyt solution by rotating the device in the solution ten (10) times while pushing against the PreservCyt vial wall. Swirl the brush vigorously to further release material. Discard the brush.

2. Tighten the cap so thet the trogue line on the cap passes thru the trogue line on the vial.

3. Record the patient's name and date of brith on the vial. The vial must contain two (2) patient idenfiers

4. Record the following patient information on a Cytology/PAP requisition (form #320-1407).

  a. Patient name

  b. Date of birth

  c. Gender

  d. Medical record or social security number

  e.Physician name

  f. Date of collection

  g. Source of material

  h. Clinical information

  i. Patient history

5. Place the vial and requisition in a biohazard bag for transport to the laboratory.

6. The following specimens will be returned to submitting provider office:

  a. Spilled/leaking specimen vial

  b. No requisition received with vial

  c. Unlabeled specimen vial

  d. Name on requisition form does not match name on specimen vial

  e. Specimen collected in formalin.

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