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HelpTest Code RAIFA Antinuclear Antibodies, HEp-2 Substrate, IgG, with Reflex, Serum
Ordering Guidance
This algorithm is recommended for the initial evaluation of patients at risk for systemic lupus erythematosus, mixed connective tissue disease, and Sjogren syndrome.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.7 mL Serum
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Evaluation of patients suspected of having systemic autoimmune rheumatic disease (antinuclear antibody-associated rheumatic diseases or connective tissue diseases), especially systemic lupus erythematosus, mixed connective tissue disease and Sjogren syndrome
Highlights
This test ensures that testing proceeds in an algorithmic fashion based on the presence of homogeneous, speckled or dense fine speckled patterns.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RFLXP | ANA Ab HEp-2 Reflex Panel | No | No |
Testing Algorithm
If the human epithelial type 2 (HEp-2) antinuclear antibodies indirect immunofluorescence assay (IFA) result is positive with a titer of 1:80 or greater, then a titer and pattern will be reported.
If positive for a homogeneous, speckled, or dense fine speckled pattern, then confirmatory testing for double-stranded DNA antibodies (Ab), ribonucleoprotein Ab, Scl-70 Ab, Sm Ab, SS-A/Ro Ab, or SS-B/La Ab will be reflexed and performed at an additional charge. If confirmatory tests are negative, consideration of other associated antinuclear antibodies may be required for evaluation and confirmatory testing for these may be performed based on reported patterns or clinical suspicion at an additional charge.
Method Name
Indirect Immunofluorescence Assay (IFA)
Reporting Name
Antinuclear Ab, HEp-2,with reflex,SSpecimen Type
SerumSpecimen Minimum Volume
Serum: 0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 21 days |
| Frozen | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-treated specimen | Reject |
Reference Values
<1:80 (negative)
Day(s) Performed
Monday through Saturday
Report Available
3 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86039
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| RAIFA | Antinuclear Ab, HEp-2,with reflex,S | 59069-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| ANAH | Antinuclear Ab, HEp-2 Substrate, S | 59069-5 |
| 1TANA | ANA Titer: | 33253-6 |
| 1PANA | ANA Pattern: | 49311-4 |
| 2TANA | ANA Titer 2: | 33253-6 |
| 2PANA | ANA Pattern 2: | 49311-4 |
| CYTQL | Cytoplasmic Pattern: | 55171-3 |
| LCOM | Lab Comment: | 77202-0 |