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Test Code RFLXP Antinuclear Antibodies, HEp-2, Reflex Panel


Specimen Required


Only orderable as a reflex. For more information see RAIFA / Antinuclear Antibodies, HEp-2 Substrate, IgG, with Reflex, Serum.

 

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.7 mL Serum

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Confirmatory testing after a positive homogenous, speckled, or dense fine speckled pattern identified in the human epithelial type 2 antinuclear antibody indirect immunofluorescence assay

Profile Information

Test ID Reporting Name Available Separately Always Performed
ADNA1 dsDNA Ab, IgG, S Yes Yes
RNP RNP Ab, IgG, S Yes Yes
SCL70 Scl 70 Ab, IgG, S Yes Yes
SM Sm Ab, IgG, S Yes Yes
SSA SS-A/Ro Ab, IgG, S Yes Yes
SSB SS-B/La Ab, IgG, S Yes Yes
IM_04 Antinuclear Ab,HEp-2,reflex Comment No No

Method Name

Only orderable as a reflex. For more information see RAIFA / Antinuclear Antibodies, HEp-2 Substrate, IgG, with Reflex, Serum.

 

ADNA1: Enzyme-Linked Immunosorbent Assay (ELISA)

RNP, SCL70, SM, SSA, SSB: Multiplex Flow Immunoassay

IM_04: Technical Interpretation

Reporting Name

ANA Ab HEp-2 Reflex Panel

Specimen Type

Serum

Specimen Minimum Volume

Serum: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK
Heat-treated specimen Reject

Reference Values

Only orderable as a reflex. For more information see RAIFA / Antinuclear Antibodies, HEp-2 Substrate, IgG, with Reflex, Serum.

 

DOUBLE-STRANDED DNA (dsDNA) ANTIBODIES, IGG, SERUM

<100 IU/mL (negative)

≥100 IU/mL (positive)

Negative is considered normal.

Reference values apply to all ages.

 

RNP ANTIBODIES, IGG, SERUM

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

 

SCL 70 ANTIBODIES, IGG, SERUM

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

 

SMITH (Sm) ANTIBODIES, IGG, SERUM

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

 

SS-A/RO ANTIBODIES, IGG, SERUM

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

 

SS-B/LA ANTIBODIES, IGG, SERUM

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Day(s) Performed

Monday through Saturday

Report Available

3 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

Not Applicable

CPT Code Information

86225

86235 x5

LOINC Code Information

Test ID Test Order Name Order LOINC Value
RFLXP ANA Ab HEp-2 Reflex Panel 97564-9

 

Result ID Test Result Name Result LOINC Value
ADNA1 dsDNA Ab, IgG, S 33799-8
IM_04 Antinuclear Ab,HEp-2,reflex Comment 77202-0
RNP RNP Ab, IgG, S 29958-6
SCL70 Scl 70 Ab, IgG, S 47322-3
SM Sm Ab, IgG, S 18323-6
SSA SS-A/Ro Ab, IgG, S 33610-7
SSB SS-B/La Ab, IgG, S 33613-1