Test Code WBCD White Blood Count (WBC) Differential, Blood
Performing Laboratory
MaineGeneral Medical Center
Specimen Requirements
Specimen must arrive within 24 hours of draw.
Capillary specimens collected in a MAP microtube must be tested within 4 hours of collection.
Keep specimens refrigerated.
Whole Blood
Draw blood in a lavender-top (K2-EDTA) tube. For pediatric specimens, collect blood in a lavender-top BD Microtainer® MAP Microtube. Invert several times to mix and to avoid clotting. Label the specimen with the patient’s name (first and last), date of birth, date and time of draw and the phlebotomist’s initial’s. Send whole blood refrigerated.
*Collection tube must be filled to ½ of its intended total volume to ensure adequate blood to anticoagulant ratio.
Specimen stability:
24 hours at room temperature or refrigerated / 4 hours at room temperature if capillary
*Smear preparation on specimens older than 4 hours may exhibit a loss of cellular integrity.
Minimum volume:
500 µL
Specimen rejection criteria:
Clotted blood
Specimens that are >24 hours venous or >4 hours capillary
Collection tube is less than one-half its intended volume
Improper patient identification
Day(s) Test Performed
Monday - Sunday
Test Classification and CPT Coding
85007 - differential
85048 - WBC
Reference Values
WBC
0-8 days: 6.0-25.0 K/µL
9 days-24 months: 5.0-14.0 K/µL
2-9 years: 4.8-10.8 K/µL
10-17 years: 4.8-10.8 K/µL
≥18 years: 4.8-10.8 K/µL
Critical values (automatic call-back): <1.0 K/µL or >50.0 K/µL
LYMPHOCYTES
0-8 days: 14-46%
9 days-9 years: 20-60%
≥10 years: 20-40%
MONOCYTES
0-8 days: 1-15%
≥9 days: 4-12%
GRANULOCYTES
0-8 days: 27-71%
9 days-9 years: 30-70%
≥10 years: 55-75%
EOSINOPHILS
1-4%
BASOPHILS
0-1%